Stochastic Optical Reconstruction Microscopy (STORM) Literature References
Stochastic optical reconstruction microscopy (STORM) relies on the sequential and stochastic readout with precise localization of multiple single molecules from specimens that were fluorescently labeled with photoswitchable dyes at densities approaching those commonly employed in widefield and confocal fluorescence microscopy (ranging up to 100,000 molecules per square micrometer). STORM utilizes an activation laser applied at low power to trigger emission switching so that any particular molecule has a small probability of being photoactivated, but the majority of the population remains in the original (dark or native) emissive state.
Recommended Literature
- Bates, M., Huang, B., Dempsey, G. T. and Zhuang, X. Multicolor super-resolution imaging with photo-switchable fluorescent probes. Science 317: 1749-1753 (2007).
- Bates, M., Huang, B. and Zhuang, X. Super-resolution microscopy by nanoscale localization of photo-switchable fluorescent probes. Current Opinion in Chemical Biology 12: 505-514 (2008).
- Henriques, R. and Mhlanga, M. M. PALM and STORM: What hides beyond the Rayleigh limit? Biotechnology Journal 4: 846-857 (2009).
- Huang, B. Super-resolution optical microscopy: Multiple choices. Current Opinion in Chemical Biology 14: 10-14 (2010).
- Huang, B., Jones, S. A., Brandenburg, B. and Zhuang, X. Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution. Nature Methods5: 1047-1052 (2008).
- Rust, M. J., Bates, M. and Zhuang, X. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nature Methods 3: 793-796 (2006).
- Zhuang, X. Nano-imaging with STORM. Nature Photonics 3: 365-367 (2009).
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