RESOLFT Concept Literature References

The RESOLFT concept was introduced by Stefan Hell to describe breaking the diffraction barrier in far-field microscopy through the use of switchable or reversibly saturable fluorophore transitions. Professor Hell first described RESOLFT (REversible Saturable or switchable OpticaL Fluorescence Transitions) in terms of stimulated emission depletion (STED) and ground state depletion (GSD) microscopy, where the diffraction barrier is broken by a saturated optical transition (depletion) between two states of a fluorescent probe. Although the concept of RESOLFT was originally developed using techniques that rely on point-spread function engineering, it has also been utilized to describe superresolution methodologies that rely on localization of single molecules.