Epi-Fluorescence filter combinations are housed in a filter cube and include an excitation filter, dichroic mirror (or beamsplitter), and a barrier filter. Ultraviolet and visible transmission spectra for the Nikon Endow GFP Bandpass Emission (FGP(R)-BP) filter cube are illustrated below.
Endow GFP Bandpass Emission (FGP(R)-BP)
- Excitation Wavelength: 450-490 nanometers (470 CWL)
- Dichroic Mirror Wavelength: 495 nanometers (LP)
- Barrier Wavelength: 500-550 nanometers (525 CWL)
The Endow GFP Bandpass Emission (FGP(R)-BP) filter combination is a bandpass version of the Endow GFP longpass filter set and was designed by Dr. Sharyn Endow of Duke University. This filter set is useful for fluorescence studies utilizing wtGFP and EGFP. Along with other filters in this series, the set is designed to increase secondary fluorescence intensity (brightness) without a consequential increase in noise levels. Steeper passbands are created by increasing the number of interference cavities in both the excitation and emission filters, allowing close proximity between filter spectra. The resulting wider and steeper filter spectra allow the set to provide more excitation energy and capture more fluorescence intensity.