Epi-Fluorescence filter combinations are housed in a filter cube and include an excitation filter, dichroic mirror (or beamsplitter), and a barrier filter. Ultraviolet and visible transmission spectra for the Nikon Endow GFP Longpass Emission (FGP(R)-LP) filter cube are illustrated below.
- Excitation Wavelength: 450-490 nanometers (470 CWL)
- Dichroic Mirror Wavelength: 495 nanometers (LP)
- Barrier Wavelength: 500 nanometers (LP)
The Endow GFP longpass emission (FGP(R)-LP) filter combination is a longpass version of the Endow GFP bandpass filter set and was designed by Dr. Sharyn Endow of Duke University. This filter set is useful for fluorescence studies utilizing wtGFP and EGFP. Along with other filters in this series, the set is designed to increase secondary fluorescence intensity (brightness) without a consequential increase in noise levels. Steeper passbands are created by increasing the number of interference cavities in both the excitation and emission filters, allowing close proximity between filter spectra. The resulting wider and steeper filter spectra allow the set to provide more excitation energy and capture more fluorescence intensity.