Epi-Fluorescence filter combinations are housed in a filter cube and include an excitation filter, dichroic mirror (or beamsplitter), and a barrier filter. Ultraviolet and visible transmission spectra for the Nikon Piston GFP Bandpass Emission filter cube are illustrated below.
Piston GFP Bandpass Emission
- Excitation Wavelength: 450-490 nanometers (470 CWL)
- Dichroic Mirror Wavelength: 495 nanometers (LP)
- Barrier Wavelength: 500-530 nanometers (515 CWL)
The Piston GFP Bandpass Emission filter combination is a bandpass filter set designed by Dr. David Piston of Vanderbilt University. The emission filter has a narrow bandpass that is useful to quantitate EGFP (Endow Green Fluorescence Protein) fluorescence while avoiding autofluorescence at wavelengths exceeding 540 nanometers.