African Green Monkey Kidney Cell Actin Cytoskeleton
G-2E/C Bandpass Emission (Narrow Bandwidth Excitation) Green Set
Fluorescence emission intensity from a culture of African green monkey kidney (COS-1) cells stained with Alexa Fluor 546 conjugated to phalloidin, which binds to the intracellular filamentous actin network. The visible light absorption maximum of Alexa Fluor 546 is 556 nanometers and the emission maximum occurs at 573 nanometers (in the yellow region of the visible light spectrum). In addition, the specimen was simultaneously stained with Hoechst 33258 (targeting DNA in the cell nucleus; blue emission) and MitoTracker Green FM (targeting mitochondria; green emission). Note the absence of signal from the green (MitoTracker) and blue (Hoechst) fluorophores, which are not efficiently excited at green wavelengths, but the bright orange-red fluorescence exhibited by the actin network. This cell line does not display the prominent microfilaments typically seen with many epithelial cultures, such as Indian Muntjac, HeLa, and PtK2, but instead forms smaller fibrils that have a much smoother appearance.