African Green Monkey Kidney Cells with Propidium Iodide

TRITC HYQ Bandpass Emission (Medium Bandwidth Excitation) Green Set

Fluorescence emission intensity from a culture of transformed African green monkey kidney cells (COS-7 line) that were labeled with Alexa Fluor 350 conjugated to wheat germ agglutinin for the Golgi apparatus, Alexa Fluor 488 conjugated to phalloidin for cytoskeletal actin, and propidium iodide for the nuclei. The visible light absorption maximum of propidium iodide, a phenanthridine specific for double stranded DNA and RNA, is 536 nanometers and the emission maximum occurs at 617 nanometers. Note the lack of fluorescence signal from the blue and green fluorophores with the Nikon TRITC HYQ filter combination.

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