African Water Mongoose Skin Fibroblast Cells (A.P. Mongoose Line)
The peroxisome organelles present in the African water mongoose cell culture (APM line) shown above were immunofluorescently labeled with Alexa Fluor 750 conjugated to rabbit secondary antibody fragments directed against rabbit primary antibodies to peroxisomal membrane protein 70 (PMP 70), a major peroxisome membrane polypeptide. In addition, the culture was treated with Alexa Fluor 488 conjugated to phalloidin and MitoTracker Red CMXRos, targeting cytoskeletal F-actin and the mitochondrial network, respectively. Hoechst 33342 was employed as a nuclear counterstain. Images were recorded in grayscale with a 12-bit digital camera coupled to a Nikon Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles, with the exception of Alexa Fluor 750, which was pseudocolored yellow.