African Water Mongoose Skin Fibroblast Cells (A.P. Mongoose Line)
Focal adhesions were visualized in the log phase adherent monolayer culture of African water mongoose cells illustrated above by immunofluorescent treatment with mouse anti-vinculin primary antibodies followed by goat anti-mouse Fab fragments conjugated to Texas Red. The actin cytoskeletal network was simultaneously imaged with Alexa Fluor 488 conjugated to phalloidin, and nuclei were counterstained with Hoechst 33258. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.