African Water Mongoose Skin Fibroblast Cells (A.P. Mongoose Line)

The culture of water mongoose skin fibroblast cells that is presented in the digital image above was labeled with MitoTracker Red CMXRos and Alexa Fluor 488 conjugated to phalloidin, which target mitochondria and filamentous actin, respectively. In addition, the cells were stained for nuclear DNA with Hoechst 33258. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.