African Water Mongoose Skin Fibroblast Cells (A.P. Mongoose Line)

The microtubule network of the adherent culture of A.P. Mongoose fibroblast cells presented in the digital image above was immunofluorescently labeled with anti-tubulin mouse monoclonal primary antibodies followed by goat anti-mouse secondary antibodies (IgG) conjugated to Alexa Fluor 568 (yielding red emission). In addition, the cells were labeled for cytoskeletal F-actin with Alexa Fluor 488 (green emission) conjugated to phalloidin, and for cell nuclei with Hoechst 33342. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.