Alfalfa Root Thin Section
FITC-Texas Red Bandpass Emission (Dual Band Excitation) Set
Autofluorescence emission intensity from a thin section of alfalfa (Medicago sativa) root tissue. Endogenous autofluorescence in plant tissues arises from a variety of biomolecules, including lignins, chlorophyll, carotene, and xanthophyll. In the blue and green excitation regions, chlorophyll has an absorption band with a high extinction coefficient and produces a significant amount of fluorescence when excited with wavelengths between 450 and 550 nanometers. For the alfalfa root tissue illustrated above, note the presence of autofluorescence emission intensity in the green and red spectral regions, which is characteristic of the Nikon FITC-Texas Red fluorescence filter combination.