Bovine Pulmonary Artery Cell Actin

B-1E Bandpass Emission (Narrow Bandwidth Excitation) Blue Set

Fluorescence emission intensity from a culture of bovine pulmonary artery endothelial cells stained with BODIPY FL phallacidin, which binds to the intracellular filamentous actin network. The visible light absorption maximum of BODIPY FL is 503 nanometers and the emission maximum occurs at 512 nanometers. In addition, the specimen was simultaneously stained with DAPI (targeting DNA in the cell nucleus; blue emission) and MitoTracker Red CMXRos (targeting mitochondria; red emission). Note the absence of signal from the red (MitoTracker) and blue (DAPI) fluorophores, but the bright green fluorescence exhibited by the actin filaments.