Bovine Pulmonary Artery Cell Nuclei, Actin, and Tubulin
DAPI-FITC-TRITC Bandpass Emission (Triple Band Excitation) Set
Fluorescence emission intensity from a culture of bovine pulmonary artery endothelial cells that were immunofluorescently labeled with primary anti-bovine alpha-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to BODIPY FL. The cell culture was simultaneously stained with Texas Red phalloidin and DAPI, which target the cytoskeletal actin network and DNA in the nucleus, respectively. The visible light absorption maximum of BODIPY FL is 505 nanometers and the emission maximum occurs at 513 nanometers, while the corresponding values for Texas Red are 595 and 620 nanometers. DAPI (4',6-diamidino-2-phenylindole) is an ultraviolet light-absorbing dye with an absorption maximum at 358 and an emission maximum in the blue region at 461 nanometers. Note the presence of high signal levels from DAPI and BODIPY FL, but the relatively low signal exhibited by Texas Red (the DAPI-FITC-TRITC filter combination does not efficiently gather signal at longer red wavelengths).