Bovine Pulmonary Artery Cell Tubulin
G-1B Longpass Emission (Narrow Bandwidth Excitation) Green Set
Fluorescence emission intensity from a culture of bovine pulmonary artery endothelial cells that were immunofluorescently labeled with primary anti-bovine alpha-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 546. The visible light absorption maximum of Alexa Fluor 546 is 556 nanometers and the emission maximum occurs at 573 nanometers (in the yellow region of the visible light spectrum). Note the prominent orange-red staining of the intracellular microtubule network that extends throughout the cytoplasm.