Bovine Pulmonary Artery Endothelial Cells (BPAE Line)

The bovine pulmonary artery endothelial cells that appear in the digital image above were resident in a BPAE cell culture that was stained with MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and Hoechst 33342, which target the mitochondrial network, filamentous actin, and DNA in the nucleus, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to a Nikon Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.