Bovine Pulmonary Artery Endothelial Cells (BPAE Line)
The culture of BPAE cells illustrated above was labeled for the cytoskeletal filamentous actin network with Alexa Fluor 488 conjugated to phalloidin, and for the cell nucleus with Hoechst 33342. Additionally, cellular mitochondria were stained with MitoTracker Red CMXRos, a complex aminated xanthene derivative. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.