Bovine Pulmonary Artery Endothelial Cells (BPAE Line)

Alexa Fluor 488 conjugated to soybean agglutinin, a lectin isolated from Glycine max, was utilized to immunofluorescently label terminal alpha- and beta-N-acetylgalactosamine and galactopyranosyl residues in a culture of bovine pulmonary artery endothelial cells. The specimen was also stained with Texas Red conjugated to phalloidin and DAPI to visualize filamentous actin and cell nuclei, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.