Bovine Pulmonary Artery Endothelial Cells (BPAE Line)

The intracellular mitochondrial network of the culture of bovine pulmonary artery endothelial cells appearing in the digital image above was stained with MitoTracker Red CMXRos. The specimen was also labeled for filamentous actin and DNA with Alexa Fluor 488 (green emission) conjugated to phalloidin and DAPI (blue emission), respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.