Bovine Pulmonary Artery Endothelial Cells (BPAE Line)

Presented in the digital image above is an adherent culture of BPAE cells that was labeled with Alexa Fluor 488 conjugated to soybean agglutinin, a lectin isolated from Glycine max that selectively binds terminal alpha- and beta-N-acetylgalactosamine and galactopyranosyl residues. In addition, the cells were labeled with Texas Red conjugated to phalloidin and DAPI, targeting the cytoskeletal F-actin network and DNA, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

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