Bovine Pulmonary Artery Endothelial Cells (BPAE Line)

Applying a popular triple fluorophore technique, the BPAE cell culture presented above was stained with MitoTracker Red CMXRos, DAPI, and Alexa Fluor 488 conjugated to phalloidin, which target the mitochondrial network, nuclear DNA, and F-actin, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.