Canine Kidney Cells with Alexa Fluor 488 and MitoTracker Red CMXRos

FITC-Texas Red Bandpass Emission (Dual Band Excitation) Set

Fluorescence emission intensity from a culture of Madin-Darby canine kidney (MDCK line) cells stained with MitoTracker Red CMXRos and Alexa Fluor 488-phalloidin, which target the intracellular mitochondrial network and cytoskeletal actin filaments, respectively. The visible light absorption maximum of MitoTracker Red CMXRos is 579 nanometers and the emission maximum occurs at 599 nanometers, while the corresponding values for Alexa Fluor 488 are 498 and 519 nanometers. In addition, the specimen was simultaneously stained with DAPI (targeting DNA in the cell nucleus; blue emission). Note the absence of signal from the blue (DAPI) fluorophore, but the bright orange-red fluorescence exhibited by the tubular mitochondria and the green emission from actin filaments in the cytoplasm.