Canine Kidney Cells with DAPI, Alexa Fluor 568, and FITC
DAPI-FITC-TRITC Bandpass Emission (Triple Band Excitation) Set
Fluorescence emission intensity from a culture of canine kidney epithelial cells (Madin-Darby; MDCK line) that were immunofluorescently labeled with primary anti-human golgin-97 mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to fluorescein (through the isothiocyanate, FITC). The visible light absorption maximum of FITC is 494 nanometers and the emission maximum occurs at 518 nanometers in the blue-green region of the spectrum. In addition, the specimen was simultaneously stained with DAPI (targeting DNA in the cell nucleus; blue emission) and Alexa Fluor 568 conjugated to phalloidin (targeting the cytoskeletal actin network; orange-red emission). Anti-human golgin-97 antibodies recognize a 97-kiloDalton protein termed golgin-97, which is a member of the granin protein family and a peripheral membrane protein localized on the cytoplasmic face of the Golgi apparatus. Note the presence of green mitochondria in this specimen.