Canine Kidney Epithelial Cell F-Actin Network

TRITC HYQ Bandpass Emission (Medium Bandwidth Excitation) Green Set

Fluorescence emission intensity from a culture of canine kidney epithelial cells (Madin-Darby; MDCK line) stained with phalloidin conjugated to Alexa Fluor 568, which targets the intracellular cytoskeletal F-actin network. Phalloidins are a member of the phallotoxin group of bicyclic peptides isolated from the deadly Amanita phalloides mushroom. The visible light absorption maximum of Alexa Fluor 568 is 578 nanometers and the emission maximum occurs at 603 nanometers. Note the bright fluorescence signal exhibited by the filamentous actin network, which extends throughout the cytoplasm. In addition to phalloidin, the specimen was also labeled with wheat germ agglutinin conjugated to Oregon Green 488 (targeting the Golgi network; blue excitation and green emission) and DAPI (staining DNA in the nucleus; ultraviolet excitation and blue emission). Neither of these fluorophores are imaged with the Nikon TRITC HYQ filter combination.