Caster Bean Thin Section

Texas Red HYQ Bandpass Emission (Wide Bandwidth Excitation) Yellow Set

Autofluorescence emission intensity from a thin section of caster bean (Ricinus communis) tissue. Endogenous autofluorescence in plant tissues arises from a variety of biomolecules, including chlorophyll, carotene, and xanthophyll. In the yellow excitation region, chlorophyll has an absorption band with a low extinction coefficient, but still produces a detectable level of fluorescence at emission wavelengths of 580 nanometers and above (orange and red). For the caster bean thin section illustrated above, note the presence of autofluorescence emission intensity in the red spectral regions, but the absence of intensity at lower (green and blue) wavelengths.

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