Chinese Hamster Ovary Cells (CHO-K1 Line)

A culture of CHO-K1 cells (illustrated above) was labeled with a triplet of fluorophores, including MitoTracker Orange CMTM Ros, Oregon Green 488, and Hoechst 33258. The Oregon Green probe was conjugated to phalloidin in order to target filamentous actin. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.