Embryonic Swiss Mouse Fibroblast Cells (3T3 Line)

The mitochondrial network and nuclear DNA were imaged in a culture of 3T3 fibroblast cells with MitoTracker Red CMXRos and Hoechst 33258, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

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