Embryonic Swiss Mouse Fibroblast Cells (3T3 Line)
The culture of embryonic Swiss mouse fibroblast cells that is presented in the digital image above was labeled with SYTOX Orange and Alexa Fluor 488 conjugated to phalloidin to target nuclear DNA and filamentous actin, respectively. In addition, mitochondria were labeled with MitoTracker Deep Red 633. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles with the exception of MitoTracker Deep Red 633, which was pseudocolored yellow.