Embryonic Swiss Mouse Fibroblast Cells (3T3 Line)

The 3T3 cell culture presented above was immunofluorescently labeled with mouse anti-alpha-tubulin primary antibodies followed by goat anti-mouse secondary antibodies conjugated to the cyanine dye Cy3. In addition, filamentous actin in the culture was targeted with Alexa Fluor 488 conjugated to phalloidin and cell nuclei were labeled with DAPI. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.