Embryonic Swiss Mouse Fibroblast Cells (3T3 Line)
The culture of embryonic Swiss mouse fibroblast cells presented in the digital image above was labeled with MitoTracker Red CMXRos and Alexa Fluor 488 conjugated to phalloidin, targeting the mitochondrial network and filamentous actin, respectively. The culture was counterstained for DNA in the cell nucleus with DAPI. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.