Embryonic Swiss Mouse Fibroblast Cells (3T3 Line)

The culture of 3T3 cells featured in the image above was labeled for peroxisomes with Rhodamine Red conjugated to goat secondary antibodies that target rabbit anti-PMP 70 (peroxisomal membrane protein 70). The culture was additionally labeled with Alexa Fluor 488 conjugated to phalloidin and Hoechst 33258, targeting filamentous actin and the nucleus, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.