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Fibroblasts with Alexa Fluor 568 and LysoTracker DND-26

FITC-Texas Red Bandpass Emission (Dual Band Excitation) Set

Fluorescence emission intensity from a culture of Indian Muntjac deerskin fibroblast cells stained with Alexa Fluor 568 conjugated to phalloidin and LysoTracker Green DND-26, which target intracellular actin and lysosomes, respectively. The visible light absorption maximum of Alexa Fluor 568 is 578 nanometers and the emission maximum occurs at 603 nanometers, while the corresponding values for LysoTracker Green DND-26 are 501 and 511 nanometers. In addition, the specimen was simultaneously stained with DAPI (targeting DNA in the cell nucleus; excitation at 358 nanometers and emission at 461 nanometers). Note the absence of signal from the blue fluorophore (DAPI), but the presence of bright green and orange-red fluorescence exhibited by the lysosomes and actin filaments in the cytoskeletal network.

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