Golgi Complex Labeled with EGFP
Membrane-bound stacks known as cisternae are the foundation of the Golgi complex. Between five and eight stacks are usually present; however, as many as sixty have been observed. The cisternae stack has five functional regions: the cis-Golgi network, cis-Golgi,medial-Golgi, trans-Golgi, and trans-Golgi network. Vesicles from the endoplasmic reticulum fuse with the cis-Golgi network and subsequently progress through the stack to thetrans-Golgi network, where they are packaged and sent to the required destination. Each region contains different enzymes that selectively modify the contents depending on where they are destined to reside. In the digital videos presented above, opossum kidney epithelial cells (OK line) are visualized expressing a fusion of enhanced green fluorescent protein (EGFP) with a targeting signal to localize the chimera to the Golgi apparatus.