Golgi Complex Labeled with EYFP
Proteins, phospholipids, and carbohydrates, along with other molecules formed in the endoplasmic reticulum are transported to the Golgi apparatus where they are biochemically modified during their transition from the cis to the trans poles of the complex. Enzymes present in the Golgi lumen modify the carbohydrate (or sugar) portion of glycoproteins by adding or subtracting individual sugar monomers. In addition, the Golgi apparatus manufactures a variety of macromolecules on its own, including a variety of polysaccharides. The Golgi complex in plant cells produces pectins and other polysaccharides specifically needed for plant structure and metabolism. The products exported by the Golgi apparatus through the trans face eventually fuse with the plasma membrane of the cell. In the digital videos presented above, normal opossum kidney epithelial cells (OK line) are observed expressing enhanced yellow fluorescent protein (EYFP) fused to a Golgi complex targeting signal.