Gray Fox Lung Fibroblast Cells Expressing YPet Fluorescent Protein-EB3

Microtubules are biopolymers that are composed of subunits made from an abundant globular cytoplasmic protein known as tubulin. Each subunit of the microtubule is made of two slightly different but closely related simpler units called alpha-tubulin and beta-tubulin that are bound very tightly together to form heterodimers. In a microtubule, the subunits are organized in such a way that they all point the same direction to form 13 parallel protofilaments. This organization gives the structure polarity, with only the alpha-tubulin proteins exposed at one end and only beta-tubulin proteins at the other. The digital videos presented in this section examine the tracking of microtubule +TIPs (plus end tracking proteins) in Gray fox lung fibroblast cells labeled with a chimera of EB3 (a microtubule end-binding protein) fused to YPet (yellow) fluorescent protein

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