Grey Fox Lung Fibroblast Cells (FoLu Line)

The culture of fox lung fibroblast cells presented in the digital image above was labeled for the mitochondrial and filamentous actin networks with MitoTracker Deep Red 633 and Alexa Fluor 568 (red fluorescence) conjugated to phalloidin, respectively. DNA in the cell nucleus was counterstained with SYTOX Green. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles with the exception of MitoTracker Deep Red 633, which was pseudocolored yellow.