Horse Dermal Fibroblast Cells (NBL-6 Line)
Mitochondria and the cytoskeletal actin framework were visualized in a culture of NBL-6 cells (shown above) with MitoTracker Red CMXRos and Alexa Fluor 633 conjugated to phalloidin. Cell nuclei were counterstained with SYTOX Green. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, instead of individual image channels being pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles, Alexa Fluor 633 was pseudocolored purple, MitoTracker Red CMXRos was pseudocolored green, and SYTOX Green was pseudocolored cyan.