HeLa Cell Mitochondria

G-1B Longpass Emission (Narrow Bandwidth Excitation) Green Set

Fluorescence emission intensity from a culture of HeLa epithelial cells stained with MitoTracker Red CMXRos, which targets the intracellular mitochondrial network. The visible light absorption maximum of MitoTracker Red CMXRos is 579 nanometers and the emission maximum occurs at 599 nanometers. In addition, the specimen was simultaneously stained with DAPI (binding to DNA in the cell nucleus; blue emission) and Alexa Fluor 488 conjugated to phalloidin (targeting actin; green emission). Note the absence of signal from the blue and green fluorophores, which are not efficiently excited by the narrow passband of the G-1B filter combination. However, the relatively bright yellow-orange fluorescence exhibited by the tubular mitochondria is quite evident in the image.