Human Bone Osteosarcoma Cells (U-2 OS Line)

F-actin, mitochondria, and cell nuclei were fluorescently labeled in the culture of U-2 OS cells presented in the digital image above with MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DAPI, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

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