Human Bone Osteosarcoma Cells (U-2 OS Line)

The relationship between the F-actin cytoskeletal framework and the mitochondrial network was visualized in the culture of human osteosarcoma cells presented in the digital image above with the green fluorescent dye Alexa Fluor 488 conjugated to phalloidin and MitoTracker Red CMXRos, respectively. DNA in the cell nucleus was targeted with Hoechst 33342. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.