Human Bone Osteosarcoma Cells (U-2 OS Line)

The culture of osteosarcoma cells (U-2 OS line) depicted in the digital image above was labeled with MitoTracker Red CMXRos (red emission) and Alexa Fluor 488 (green emission) conjugated to phalloidin, targeting the mitochondrial network and the cytoskeletal actin framework, respectively. The culture was counterstained with DAPI, which fluoresces blue upon binding to AT regions of DNA (labeling the nucleus). Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.