Human Bone Osteosarcoma Cells (U-2 OS Line)

The proximity of the intracellular mitochondrial and filamentous actin networks was visualized in a culture of human osteosarcoma cells (illustrated above) with MitoTracker Red CMXRos and Alexa Fluor 488 conjugated to phalloidin. DNA in the cell nucleus was counterstained with 4',6-diamidino-2-phenylindole (DAPI). Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.