Human Bone Osteosarcoma Cells (U-2 OS Line)

The adherent culture of human osteosarcoma cells (U-2 OS line) featured in the digital image above was transfected with a pDsRed-Mitochondria plasmid subcellular localization vector, thus localizing a red fluorescent protein tag to the intracellular mitochondrial network. The culture was also immunofluorescently labeled with mouse anti-alpha-tubulin followed by a secondary antibody (goat anti-mouse IgG) conjugated to Marina Blue to target microtubules. Cell nuclei were counterstained with SYTOX Green. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.