Human Cervical Adenocarcinoma Cells (HeLa Line)

An adherent culture of human cervical adenocarcinoma cells (HeLa line) was fixed, permeabilized, treated with sodium borohydride to reduce unreacted aldehydes, and then blocked with 10-percent normal goat serum in phosphate buffered saline before treatment with anti-tubulin mouse monoclonal primary antibodies followed by goat anti-mouse secondary antibody fragments conjugated to Alexa Fluor 568. The culture was counterstained with TO-PRO-3, targeting the nucleus. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.