Human Cortical Neuronal Cells (HCN-1A Line)
Mitochondria and filamentous actin in the culture of HCN-1A cells presented in the digital image above were simultaneously labeled with MitoTracker Red CMXRos and Alexa Fluor 488 conjugated to phalloidin, respectively. Nuclear DNA was counterstained with Hoechst 33258. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.