Human Fetal Lung Fibroblast Cells (MRC-5 Line)
The peroxisomes and histones present in the MRC-5 fibroblast cell culture illustrated in the digital image above were immunofluorescently labeled with Alexa Fluor 488 conjugated to goat secondary antibody fragments directed against rabbit primary antibodies to peroxisomal membrane protein 70 (PMP 70) and Texas Red conjugated to secondary antibody fragments against mouse primary antibodies to nuclear histone proteins. In addition, the culture was labeled for F-actin with Alexa Fluor 350 conjugated to phalloidin. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.