Human Fetal Lung Fibroblast Cells (MRC-5 Line)
The culture of human fetal lung fibroblast cells presented above was stained with Alexa Fluor 350 conjugated to the lectin concanavalin A, which selectively binds to alpha-mannopyranosyl and alpha-glucopyranosyl residues (primarily in the endoplasmic reticulum). Alexa Fluor 568 conjugated to phalloidin and SYTOX Green were also used to label the culture, targeting filamentous actin and nuclear DNA, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.