Human Lung Carcinoma Cells (A-549 Line)

The culture of human lung carcinoma cells featured above was immunofluorescently labeled with primary anti-cytokeratin (pan) mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 488. In addition, the specimen was treated with MitoTracker Red CMXRos and Hoechst 33342 to stain the mitochondrial network and nuclei, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.