Human Lung Carcinoma Cells (A-549 Line)
The A-549 cell culture presented above was immunofluorescently labeled with primary anti-cytokeratin (an intermediate filament protein) mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Marina Blue. MitoTracker Red CMXRos (mitochondria) and SYTOX Green (nuclei) were also used to counterstain the carcinoma cell culture. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.