Human Osteosarcoma Cell Nuclei, Mitochondria, and Actin

DAPI-FITC-TRITC Bandpass Emission (Triple Band Excitation) Set

Fluorescence emission intensity from a culture of human osteosarcoma cells (U-2 line) stained with MitoTracker Red CMXRos and Alexa Fluor 488 conjugated to phalloidin, which target the intracellular mitochondrial network and cytoskeletal actin filaments, respectively. The visible light absorption maximum of MitoTracker Red CMXRos is 579 nanometers and the emission maximum occurs at 599 nanometers, while the corresponding values for Alexa Fluor 488 are 495 and 519 nanometers. In addition, the specimen was simultaneously stained with DAPI (targeting DNA in the cell nucleus; ultraviolet excitation with blue emission). Note the remarkable symmetry in these four daughter cells, which is clearly revealed by the presence of high signal levels from all three of the fluorophores employed to stain the specimen.